SENSITIVITY OF THE PLATELET IMMUNOFLUORESCENCE TEST (PIFT) AND THE MAIPA ASSAY FOR THE DETECTION OF PLATELET-REACTIVE ALLOANTIBODIES - A REPORT ON 2 UK NATIONAL PLATELET WORKSHOP EXERCISES

被引：8

作者：

ALLEN, DL

CHAPMAN, J

PHILLIPS, PK

OUWEHAND, WH

机构：

[1] ST BARTHOLOMEWS HOSP,LONDON EC1A 7BE,ENGLAND

[2] UNIV CAMBRIDGE,DIV TRANSFUS MED,CAMBRIDGE,ENGLAND

[3] REG BLOOD TRANSFUS CTR,OXFORD,ENGLAND

[4] NIBSC,DIV HAEMATOL,POTTERS BAR EN6 3QG,ENGLAND

来源：

TRANSFUSION MEDICINE | 1994年 / 4卷 / 02期

关键词：

MAIPA; PIFT; PLATELET ALLOANTIBODIES; PLATELET WORKSHOP;

D O I：

10.1111/j.1365-3148.1994.tb00257.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

This report presents the results of two National Workshop exercises which were designed to evaluate interlaboratory and interassay variation in sensitivity of techniques used for the detection of platelet-reactive alloantibodies. Most workshop participants used the platelet immunofluorescence test. Sensitivity of this assay was improved when fluorescence was measured by flow cytometer rather than by microscope. The MAIPA assay was found to be highly sensitive but required considerable technical expertise, and the choice of antiglycoprotein IIb/IIIa (CD61/41) monoclonal antibody was found to have a significant effect on its ability to detect anti-HPA-1a.

引用

页码：157 / 164

页数：8

共 14 条

[1] IMMUNOCHEMICAL CHARACTERIZATION OF A NEW PLATELET SPECIFIC MONOCLONAL-ANTIBODY AND ITS USE TO DEMONSTRATE THE CYTOSKELETAL ASSOCIATION OF THE PLATELET GLYCOPROTEIN-IIB-IIIA COMPLEX [J].