ASCARIS-SUUM . IMMUNOELECTROPHORETIC ANALYSIS OF ANTIGENS IN DEVELOPMENTAL STAGES

被引:8
作者
JUSTUS, DE
IVEY, MH
机构
[1] Department of Laboratory Practice, University of Oklahoma School of Health, Oklahoma City, OK
关键词
Antigenic analysis; antigenic analysis of developmental stages; Antigens; Ascaris suum; Immunoelectrophoretic analysis; stage-specific;
D O I
10.1016/0014-4894(69)90121-0
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Developmental stages of Ascaris suum and related metabolic substances (hatching fluid and larval secretions and excretions) were examined by immunoelectrophoretic analysis (IEA) for their antigenic components. A minimum of 12, 13, 14, 12, 19, and 21 antigens were found in soluble extracts of undeveloped eggs, first stage larvae (14-day embryonated eggs), infective eggs, hatched second-stage larvae, male adults, and female adults, respectively. Stage-specific antigens were identified in undeveloped eggs, first-stage larvae (eggs), infective eggs, and adults by IEA and antibody absorption. A minimum of five antigenic components were found in hatching fluid. These same components were found in unstimulated infective eggs but not in other egg stages. © 1969.
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页码:290 / &
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