REVERSAL OF STEREOSPECIFICITY OF CITRATE SYNTHASE OF CLOSTRIDIUM KLUYVERI BY P-CHLOROMERCURIBENZOATE

被引：8

作者：

OBRIEN, RW

STERN, JR

机构：

[1] Department of Pharmacology, School of Medicine Case Western Reserve University Cleveland

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1969年 / 34卷 / 03期

关键词：

D O I：

10.1016/0006-291X(69)90827-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The stereospecificity of citrate synthase in extracts of Clostridium kluyveri (Barker strain) can be changed reversibly from the (R)-type to the (S)-type by treatment with p-chloromercuribenzoate. It is proposed that sulfhydryl groups are essential for the (R)-type stereospecificity of the enzyme and their incomplete oxidation or reduction accounts for mixed labelling patterns in citrate. © 1969.

引用

页码：271 / &

共 12 条

[1]

COLOWICK SP, 1955, METHODS ENZYMOLOG ED, V1, P518

[2]

COLOWICK SP, 1957, METHODS ENZYMOLOG ED, V3, P425

[3] SYNTHESIS OF GLUTAMATE AND CITRATE BY CLOSTRIDIUM KLUYVERI . A NEW TYPE OF CITRATE SYNTHASE [J].