X-RAY INACTIVATION OF LYSOZYME IN DILUTE SOLUTION

被引：5

作者：

GORIN, G

PAPAPAVLOU, L

TAI, LW

机构：

[1] Chemistry Department, Oklahoma State University, Stillwater, OK

来源：

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY AND RELATED STUDIES IN PHYSICS CHEMISTRY AND MEDICINE | 1969年 / 15卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1080/09553006914550121

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The inactivation of lysozyme was measured in the following conditions: 40-500 μg/ml. in 0·05 M phosphate buffer, pH 6-7, 0°c, in the presence of air. The activity decreased linearly with the dose to < 25 per cent residual activity. The yield was 0·18 molecules/(100 eV). p-Aminobenzoic acid, cysteine and phenylalanine have a protective action, which parallels their rates of reaction with (OH· radicals; this indicates that (OH· is primarily responsible for inactivation of the enzyme. The meaning of the activity-dose relationship and the value of the yield are discussed. © 1969 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：33 / +

页数：1

共 25 条

[1]

[Anonymous], ENZYMES 2E

[2]

AUGENSTINE LG, 1962, ADV ENZYMOL REL S BI, V24, P359

[3] STUDIES ON THE MECHANISM OF ACTION OF IONIZING RADIATIONS .13. THE EFFECT OF X-IRRADIATION ON SOME PHYSICO-CHEMICAL PROPERTIES OF AMINO ACIDS AND PROTEINS [J].