PROPERTIES OF HYPOXANTHINEGUANINE-PHOSPHORIBOSYL-TRANSFERASE (HGPRTASE) IN A GOUT PATIENT WITH PARTIAL DEFICIENCY OF THIS ENZYME

被引:7
作者
GROBNER, W
ZOLLNER, N
机构
[1] Medizinische Poliklinik, Universität München, Dünchen 2, D-8000
来源
KLINISCHE WOCHENSCHRIFT | 1979年 / 57卷 / 02期
关键词
Gout; Hypoxanthineguanine-phosphoribosyltransferase; Partial deficiency; Physicochemical properties;
D O I
10.1007/BF01491336
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Some physicochemical properties of HGPRTase were studied in hemolysates and fibroblasts of a gout patient with partial deficiency of this enzyme. In comparison to normal HGPRTase the mutant enzyme from erythrocytes was found to have an elevated apparent Km-value for hypoxanthine and guanine and a lower Km-value for PRPP. The patient's enzyme from erythrocytes is more stable at +4°C and +80°C, the enzyme from fibroblasts more labile than that of controls. The inhibition of the mutant enzyme by some purine nucleosides and-nucleotides differed from that found in controls. No evidence was shown for an inhibitor of the patient's HGPRTase from erythrocytes. Ultracentrifugation of hemolysate in a saccharose gradient revealed no difference in the sedimentation coefficient. © 1979 Springer-Verlag.
引用
收藏
页码:63 / 68
页数:6
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