PROTEIN SYNTHESIS BY DISKS OF PUMPKIN MESOCARP

Marked synthesis of protein occurred when freshly prepared disks (1 × 10 mm) of mesocarp of ripe pumpkin (Cucurbita pepo L.) were incubated for 48 hr at 26° under moist conditions. The incubation of the disks was accompanied by an incorporation of [14C]-leucine into protein and by a 50 per cent increase in the amount of insoluble nitrogen. The intracellular distribution of [14C]-leucine incorporated into protein indicates that excision and incubation led to a steady synthesis of protein in each of the major cellular fractions. © 1970.