CONNEXON INTEGRITY IS MAINTAINED BY NONCOVALENT BONDS - INTRAMOLECULAR DISULFIDE BONDS LINK THE EXTRACELLULAR DOMAINS IN RAT CONNEXIN-43

被引：49

作者：

JOHN, SA

REVEL, JP

机构：

[1] Division of Biology 156-29 California Institute of Technology, Pasadena

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 178卷 / 03期

关键词：

D O I：

10.1016/0006-291X(91)91037-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rat heart connexin-43 (RC×43) has been isolated using a modified procedure that is rapid and can be used with fresh or frozen hearts. When RC×43 is isolated in the presence of the alkylating reagent iodoacetamide, no intermolecular disulfide bonds are found. However, the alkylated RC×43 does have at least one intramolecular disulfide bond. By using site directed antibodies and proteolytic cleavage the location of the intramolecular disulfide bonding is shown between the two extracellular loops of RC×43. © 1991.

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页码：1312 / 1318

页数：7

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