PURIFICATION AND CHARACTERIZATION OF DNA-DEPENDENT RNA-POLYMERASE FROM STAPHYLOCOCCUS-AUREUS

被引：12

作者：

DEORA, R ^{[1
]}

MISRA, TK ^{[1
]}

机构：

[1] UNIV ILLINOIS, COLL MED, DEPT MICROBIOL & IMMUNOL, CHICAGO, IL 60612 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 208卷 / 02期

关键词：

D O I：

10.1006/bbrc.1995.1382

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA dependent RNA polymerase from exponentially growing Staphylococcus aureus cells was purified. An SDS-polyacrylamide gel analysis of the most purified preparation revealed that it consists of beta, beta', alpha, and sigma with apparent molecular masses of 151, 147, 42, and 55 kDa, respectively. The sigma subunit cross reacted with a polyclonal antibody against Bacillus subtilis sigma(43). The cross reacting peptide co-migrated with the B. subtilis sigma(43). subunit. The implications of these results are discussed. Promoter specific in vitro run-off transcripts were obtained using the purified enzyme preparation. Specific conditions for the polymerization reaction are defined. (C) 1995 Academic Press, Inc.

引用

页码：610 / 616

页数：7

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