RE-EVALUATION OF CYTOCHROME C CONCENTRATIONS IN RAT ORGANS USING A NEW METHOD FOR CYTOCHROME C

被引：69

作者：

WILLIAMS, JN

THORP, SL

机构：

[1] Laboratory of Nutrition and Endocrinology, National Institute of Arthritis and Metabolic Diseases, National Institutes of Health, Bethesda

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 189卷 / 01期

关键词：

D O I：

10.1016/0005-2728(69)90220-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using a new method for analysis, the cytochrome c concentrations in rat liver, heart, brain, lung, kidney, and muscle have been determined and compared with older values reported in the literature. The validity of the procedure has been established by studying the optimal conditions for precipitation of cytochrome c, the influence of residual hemin on the calculated values for cytochrome c, recoveries of cytochrome c added before extraction from various organs and the completeness of extraction of cytochrome c from the organs. The concentrations of cytochrome c reported here for liver, brain and lung are considerably higher than those reported by earlier workers, while the values for heart, kidney and muscle are nearly the same as those reported earlier. © 1969.

引用

页码：25 / &

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