RAPID ENZYME-IMMUNOASSAY OF CHICKEN EGG-YOLK IGG

被引:2
作者
BLAIS, BW
YAMAZAKI, H
机构
[1] Department of Biology, Carleton University, Ottawa, ON
基金
加拿大自然科学与工程研究理事会;
关键词
D O I
10.3109/08820139109054927
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
A simple and rapid enzyme immunoassay for specific antibodies in chicken egg yolk is described. As a model system, the levels of anti-Salmonella IgG in the yolk of eggs obtained from various produce retailers were compared. Polyester cloth coated with Salmonella typhimurium lipopolysaccharide was used to capture specific egg yolk antibodies, which were then detected using an anti-chicken IgG-peroxidase conjugate. This assay, requiring less than 30 min to complete, revealed considerable differences in the relative levels of anti-Salmonella IgG in the egg yolks. Anti-Salmonella IgG levels were generally lower in eggs obtained from large produce retailers than in eggs obtained from a small local farm. This assay may provide a rapid and economical means of monitoring the levels of Salmonella contamination in chicken rearing facilities.
引用
收藏
页码:83 / 88
页数:6
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