MAP-BASED CLONING OF A GENE CONTROLLING OMEGA-3-FATTY-ACID DESATURATION IN ARABIDOPSIS

被引：393

作者：

ARONDEL, V

LEMIEUX, B

HWANG, I

GIBSON, S

GOODMAN, HM

SOMERVILLE, CR

机构：

[1] MICHIGAN STATE UNIV, US DOE, PLANT RES LAB, E LANSING, MI 48824 USA

[2] YORK UNIV, DEPT BIOL, N YORK M3J 1P3, ONTARIO, CANADA

[3] HARVARD UNIV, SCH MED, DEPT GENET, BOSTON, MA 02115 USA

[4] MASSACHUSETTS GEN HOSP, DEPT MOLEC BIOL, BOSTON, MA 02114 USA

来源：

SCIENCE | 1992年 / 258卷 / 5086期

关键词：

D O I：

10.1126/science.1455229

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A gene from the flowering plant Arabidopsis thaliana that encodes an omega-3 desaturase was cloned on the basis of the genetic map position of a mutation affecting membrane and storage lipid fatty acid composition. Yeast artificial chromosomes covering the genetic locus were identified and used to probe a seed complementary DNA library. A complementary DNA clone for the desaturase was identified and introduced into roots of both wild-type and mutant plants by Ti plasmid-mediated transformation. Transgenic tissues of both mutant and wild-type plants had significantly increased amounts of the fatty acid produced by this desaturase.

引用

页码：1353 / 1355

页数：3

共 24 条

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