AN NMR ASSAY FOR QUANTITATING LIPASE ACTIVITY IN BIPHASIC MACROEMULSIONS

被引：15

作者：

OCONNOR, CJ ^{[1
]}

PETRICEVIC, SF ^{[1
]}

CODDINGTON, JM ^{[1
]}

STANLEY, RA ^{[1
]}

机构：

[1] DSIR,LOWER HUTT,NEW ZEALAND

来源：

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY | 1992年 / 69卷 / 04期

关键词：

CANDIDA-CYLINDRACEA; ESTERIFICATION; ESTER SYNTHESIS; LIPASE ACTIVITY; MACROEMULSIONS; NMR ASSAY; POSITIONAL SPECIFICITY OF ENZYME REACTIONS;

D O I：

10.1007/BF02636053

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A novel nuclear magnetic resonance (NMR) assay has been developed to monitor lipase-catalyzed esterification reactions without the need to extract and purify the individual components. The technique measures ratios of ester:alcohol signals and has been shown to be both efficient and reproducible. The assay has proven useful as a quick screen for the effect of varying conditions on the extent of esterification in various biphasic solvent systems and can be applied to both saturated and unsaturated long-chain fatty acid and alcohol substrates. The NMR ratio technique has been used to quantitate the extent of reaction in the Candida cylindracea lipase-catalyzed synthesis of oleyl palmitate, stearyl palmitate, oleyl oleate, oleyl gamma-linolenate and oleyl linoleate. The identity of these products was confirmed by high-resolution mass spectrometry.

引用

页码：295 / 300

页数：6

共 11 条

[1]

Brockman H.L., 1984, P3

[2] LIPID-LIPID COMPLEXES - PROPERTIES AND EFFECTS ON LIPASE BINDING TO SURFACES [J].