RECONSTRUCTION OF HUMAN SKIN FROM GLYCEROL-PRESERVED ALLODERMIS AND CULTURED KERATINOCYTE SHEETS

被引：35

作者：

MCKAY, I

WOODWARD, B

WOOD, K

NAVSARIA, HA

HOEKSTRA, H

GREEN, C

机构：

[1] Blond McIndoe Centre for Medical Research, East Grinstead, Sussex

[2] Experimental Dermatology, London Hospital Medical College, London

[3] Euro Skin Bank, Beverwijk

[4] Surgical Research, MRC Clinical Research Centre, Middlesex

来源：

BURNS | 1994年 / 20卷

关键词：

D O I：

10.1016/0305-4179(94)90083-3

中图分类号：

R4 [临床医学];

学科分类号：

1002 ; 100602 ;

摘要：

The aim of this project was to reconstruct human skin from glycerol-preserved dermis and layers of cultured keratinocytes for use in the treatment of deep burns and ulcers. Glycerol-preserved cadaver skin from the Euro Skin Bank was treated with Dispase II or PBS, under various conditions, to find the best method of retaining the physical structure of the dermis while removing the epidermis and remnants of dead dermal cell which might provoke an allogeneic reaction in a graft recipient. Monoclonal antibodies LH39 and LH7.2, with specificity for basement membrane determinants, showed that treatment with Dispase II resulted in separation of the epidermis from the dermis with concomitant loss of all cellular elements from the dermal layer (as judged by H and E staining). However, when sheets of cultured keratinocytes were applied to the treated dermis and cultured for several days, the keratinocytes attached and regenerated a new basement membrane.

引用

页码：S19 / S22

页数：4

共 9 条

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