RELATIONSHIPS BETWEEN AMPLITUDES AND KINETICS OF RAPID CYTOSOLIC-FREE CALCIUM FLUCTUATIONS IN GH(4)C(1) RAT PITUITARY-CELLS - ROLES FOR DIFFUSION AND CALCIUM-INDUCED CALCIUM-RELEASE

被引:10
作者
BRADY, KD
WAGNER, KA
TASHJIAN, AH
GOLAN, DE
机构
[1] HARVARD UNIV,SCH PUBL HLTH,DEPT MOLEC & CELLULAR TOXICOL,BOSTON,MA 02115
[2] HARVARD UNIV,SCH MED,DEPT BIOL CHEM & MOLEC PHARMACOL,BOSTON,MA 02115
[3] BRIGHAM & WOMENS HOSP,DIV HEMATOL ONCOL,BOSTON,MA 02115
关键词
D O I
10.1016/S0006-3495(94)80961-0
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
We have examined statistical relationships between the amplitudes and the kinetics (rise times, fall times, and decay constants) of cytosolic free calcium fluctuations (spikes) in a population of 353 individual GH(4)C(1) rat pituitary cells. The fast falling phase was approximated by a single exponential decay, and the decay time constant, tau, increased linearly with spike amplitude in 80% of the cells studied. The slope of the tau versus amplitude plot for each cell was inversely related to the cell's mean spike amplitude. Thus, some process responsible for prolonging the decay phase of spikes appeared to operate strongly in cells with spikes of low amplitude, but to become less prominent in cells with high amplitude spikes. Mean tau correlated more strongly with mean rise and fall times than with mean spike amplitude, indicating that the kinetic properties of spikes were not tightly coupled to spike amplitude. These findings are consistent with a model wherein the rise phase corresponds to entry of extracellular calcium via L-type calcium channels into localized sub-plasmalemmal domains, followed by diffusion of subplasmalemmal calcium into the cell interior; and the falling phase corresponds to further calcium diffusion combined with activation of cytoplasmic calcium-induced calcium release, which prolongs the falling phase.
引用
收藏
页码:1697 / 1705
页数:9
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