FORMATION OF N-7-(2-CARBAMOYL-2-HYDROXYETHYL)GUANINE IN DNA OF THE MOUSE AND THE RAT FOLLOWING INTRAPERITONEAL ADMINISTRATION OF [C-14] ACRYLAMIDE

Acrylamide is an alkylating agent which reacts very slowly in direct reactions with DNA and is negative in the Ames test, but is carcinogenic in mice and rats. In order to explain the cancer-initiating properties of acrylamide we have studied DNA adduct formation in vitro with a metabolizing system and in vivo in mice and rats following i.p. administration of C-14-labeled acrylamide. A major adduct found in both species was N-7-(2- carbamoyl-2-hydroxyethyl)guanine, formed by reaction of the DNA with the epoxide metabolite glycidamide. The levels of this adduct were similar in the different organs of the two rodent species, which supports the notion that glycidamide is relatively evenly distributed among tissues and that the organ-specificity in acrylamide carcinogenesis cannot be explained by a selective accumulation of the DNA-reactive metabolite in target organs.