DETECTION OF CANDIDA-ALBICANS AND OTHER YEASTS IN BLOOD BY PCR

被引：89

作者：

HOLMES, AR

CANNON, RD

SHEPHERD, MG

JENKINSON, HF

机构：

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1994年 / 32卷 / 01期

关键词：

D O I：

10.1128/JCM.32.1.228-231.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Primers complementary to the region of genes coding for rRNA in Candida albicans were used in PCRs to detect yeast DNA extracted from blood samples containing various Candida specie$. One fragment (105 bp) was amplified from all yeasts tested, whereas a second (684 bp) was only amplified when C. albicans DNA was present. The level of sensitivity was 15 +/- 5 (mean +/- standard error) CFU of C. albicans per ml of blood.

引用

页码：228 / 231

页数：4

共 19 条

[1] BUCHMAN TG, 1990, SURGERY, V108, P338
[2] ISOLATION AND NUCLEOTIDE-SEQUENCE OF AN AUTONOMOUSLY REPLICATING SEQUENCE (ARS) ELEMENT FUNCTIONAL IN CANDIDA-ALBICANS AND SACCHAROMYCES-CEREVISIAE
CANNON, RD
JENKINSON, HF
SHEPHERD, MG
[J]. MOLECULAR & GENERAL GENETICS, 1990, 221 (02): : 210 - 218
[3] CANDIDA-ALBICANS-SPECIFIC AND CANDIDA-STELLATOIDEA-SPECIFIC DNA FRAGMENT
CUTLER, JE
GLEE, PM
HORN, HL
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1988, 26 (09) : 1720 - 1724
[4] DORN GL, 1978, J CLIN MICROBIOL, V7, P52
[5] INVASIVE CANDIDA INFECTIONS - EVOLUTION OF A FUNGAL PATHOGEN
EDWARDS, JE
[J]. NEW ENGLAND JOURNAL OF MEDICINE, 1991, 324 (15) : 1060 - 1062
[6] HERRMANN BG, 1987, METHOD ENZYMOL, V152, P180
[7] YEAST-SPECIFIC DNA PROBES AND THEIR APPLICATION FOR THE DETECTION OF CANDIDA-ALBICANS
HOLMES, AR
LEE, YC
CANNON, RD
JENKINSON, HF
SHEPHERD, MG
[J]. JOURNAL OF MEDICAL MICROBIOLOGY, 1992, 37 (05) : 346 - 351
[8] HOPFER RL, 1993, J MED VET MYCOL, V31, P65
[9] LABORATORY DIAGNOSIS OF INVASIVE CANDIDIASIS
JONES, JM
[J]. CLINICAL MICROBIOLOGY REVIEWS, 1990, 3 (01) : 32 - 45
[10] KANAIZUKA I, 1993, JAPAN J MED MYCOL, V34, P19

← 1 2 →