USE OF A FLAVIVIRUS RNA-DEPENDENT RNA-POLYMERASE ASSAY TO INVESTIGATE THE ANTIVIRAL ACTIVITY OF SELECTED COMPOUNDS

被引：16

作者：

BARTHOLOMEUSZ, A

TOMLINSON, E

WRIGHT, PJ

BIRCH, C

LOCARNINI, S

WEIGOLD, H

MARCUCCIO, S

HOLAN, G

机构：

[1] MONASH UNIV,DEPT MICROBIOL,CLAYTON,VIC 3168,AUSTRALIA

[2] FAIRFIELD HOSP,VICTORIAN INFECT DIS REFERENCE LAB,FAIRFIELD,VIC 3078,AUSTRALIA

[3] CSIRO,DIV CHEM & POLYMERS,CLAYTON,VIC 3168,AUSTRALIA

来源：

ANTIVIRAL RESEARCH | 1994年 / 24卷 / 04期

关键词：

HPA-23; FLAVIVIRUS; RNA-DEPENDENT RNA POLYMERASE;

D O I：

10.1016/0166-3542(94)90080-9

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

We have developed an assay using flavivirus RNA-dependent RNA polymerase to test the inhibitory activity of potential antiviral agents. The effects of antiviral agents on RNA. synthesis were examined in this assay using extracts of Vero cells infected with dengue virus type 2 or Kunjin virus. Several different classes of known polymerase inhibitors were tested. The synthesis of double-stranded replicative form RNA was inhibited in a dose-dependent fashion in the presence of the polyoxometalate HPA-23 [(NH4)(18)(NaW21Sb9O86)(17)].14 H2O and several structurally related compounds.

引用

页码：341 / 350

页数：10

共 34 条

[1] COMPARATIVE INHIBITORY EFFECTS OF SURAMIN AND OTHER SELECTED COMPOUNDS ON THE INFECTIVITY AND REPLICATION OF HUMAN T-CELL LYMPHOTROPIC VIRUS (HTLV-III) LYMPHADENOPATHY-ASSOCIATED VIRUS (LAV) [J].