PLASMA-CLEARANCE, ORGAN DISTRIBUTION AND TARGET-CELLS OF INTERLEUKIN-6 HEPATOCYTE-STIMULATING FACTOR IN THE RAT

被引：303

作者：

CASTELL, JV

GEIGER, T

GROSS, V

ANDUS, T

WALTER, E

HIRANO, T

KISHIMOTO, T

HEINRICH, PC

机构：

[1] UNIV FREIBURG, INST BIOCHEM, HERMANN HERDER STR 7, D-7800 FREIBURG, FED REP GER

[2] UNIV FREIBURG, MED KLIN 1, D-7800 FREIBURG, FED REP GER

[3] OSAKA UNIV, INST MOLEC & CELLULAR BIOL, OSAKA, JAPAN

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1988年 / 177卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1988.tb14384.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The plasma half-life of recombinant human interleukin-6 (rhIL-6) was determined in rats by measuring the disappearance of the biological activity as well as of the radioactivity of 125I-rhIL-6 from the circulation. The kinetics of clearance were biphasic. It consisted of a rapid initial disappearance corresponding to a half-life of 3 min, and of a second slow one corresponding to a half-life of about 55 min. By cellulose-acetate electrophoresis it was shown that rhIL-6 binds to a plasma protein resulting in a complex migrating in the .beta.-.gamma. region; 20 min after intravenous injection, about 80% of the 125I-rhIL-6 that had disappeared from the circulation was found in the liver. 125I-rhIL-6 was exclusively localized on the surface of parenchymal cells suggesting the existence of an interleukin-6 receptor on the hepatocytes.

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页码：357 / 361

页数：5

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