PURIFICATION OF A PHOSPHATIDYLINOSITOL 4-PHOSPHATE KINASE FROM BOVINE BRAIN MEMBRANES

被引：30

作者：

MORITZ, A

DEGRAAN, PNE

EKHART, PF

GISPEN, WH

WIRTZ, KWA

机构：

[1] STATE UNIV UTRECHT, CTR BIOMEMBRANES & LIPID ENZYMOL, PADUALAAN 8, 3584 CH UTRECHT, NETHERLANDS

[2] STATE UNIV UTRECHT, RUDOLF MAGNUS INST, DIV MOLEC NEUROBIOL, UTRECHT, NETHERLANDS

[3] STATE UNIV UTRECHT, INST MOLEC BIOL & MED BIOTECHNOL, UTRECHT, NETHERLANDS

来源：

JOURNAL OF NEUROCHEMISTRY | 1990年 / 54卷 / 01期

关键词：

Enzyme purification; Phosphatidylinositol 4‐phosphate kinase;

D O I：

10.1111/j.1471-4159.1990.tb13322.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract: A phosphatidylinositol 4‐phosphate (PIP) kinase (EC 2.7.1.68) was purified from bovine brain membranes in a six‐step procedure involving solubilization of the enzyme with 170 mM NaCl followed by chromatography on diethylaminoethyl‐cellulose, phosphocellulose, Ultrogel AcA44, hydroxylapatite, and ATP‐agarose. The enzyme preparation was nearly homogeneous and was purified 5,600‐fold with a final specific activity of 85 nmol/min/mg of protein and a yield of 20%. Its molecular mass was 110 kilodaltons, as estimated by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis. The enzyme was specific for PIP; phosphorylation of phosphatidylinositol and diacylglycerol was not observed. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：351 / 354

页数：4

共 25 条

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