ADP-RIBOSYLATION OF NUCLEAR PROTEINS IN RAT VENTRAL PROSTATE DURING AGING

被引：16

作者：

QUESADA, P ^{[1
]}

FARAONEMENNELLA, MR ^{[1
]}

JONES, R ^{[1
]}

MALANGA, M ^{[1
]}

FARINA, B ^{[1
]}

机构：

[1] AFRC, INST ANIM PHYSIOL & GENET RES, DEPT MOLEC EMBRYOL, CAMBRIDGE RES STN, CAMBRIDGE CB2 4AT, ENGLAND

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 170卷 / 02期

关键词：

D O I：

10.1016/0006-291X(90)92176-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Poly(ADPR)polymerase activity and poly(ADP-ribosyl)ation of nuclear proteins have been investigated in ventral prostate nuclei of different aged rats (14, 28, 60, 180, 360 day old animals), by reverse-phase HPLC and acetic acid-urea polyacrylamide gel electrophoresis. The major ADP-ribose acceptor proteins were identified as histone H1 and H2b. It is concluded that concomitant with major changes to chromatin organization, poly(ADP-ribosyl)ation reaction is progressively inhibited during ageing of rat ventral prostate. These results support the hypothesis that prostatic dysfunction in senescent animals is related to a failure of DNA repair mechanisms and deregulated template activity. © 1990.

引用

页码：900 / 907

页数：8

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