DETERMINATION OF SPECIFIC MONOCLONAL-ANTIBODY SECRETION RATE DURING VERY SLOW HYBRIDOMA GROWTH

被引：31

作者：

FLICKINGER, MC

GOEBEL, NK

BOHN, MA

机构：

[1] Department of Biochemistry, and Institute for Advanced Studies in Biological Process Technology, University of Minnesota, St. Paul, 55108, Minnesota

来源：

BIOPROCESS ENGINEERING | 1990年 / 5卷 / 04期

关键词：

D O I：

10.1007/BF00369579

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A total cell recycling suspension perfusion reactor has been constructed for investigation of specific monoclonal antibody secretion rate of the 9.2.27 murine hybridoma under conditions of a very low growth rate. By rapidly recycling hybridoma cells using a thermostated tangential flow filter, 3.6 mg cell dry weight/cm3 could be maintained at growth rate of <0.05 μmax for over 250 h. Under these conditions, secretion of lactate, ammonia and l-alanine were directly related to the rate of l-glutamine supply. Monoclonal antibody accumulated in the reactor to levels in excess of 1400 μg/ cm3. Surprisingly, as specific growth rate decreased, the specific immunoglobulin secretion rate remained constant, implying that monoclonal antibody synthesis could be uncoupled from growth. © 1990 Springer-Verlag.

引用

页码：155 / 164

页数：10

共 23 条

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