TOBACCO RATTLE VIRUS SEROTYPES AND ASSOCIATED NEMATODE VECTOR SPECIES OF TRICHODORIDAE IN THE BULB-GROWING AREAS IN THE NETHERLANDS

Soil samples from the coastal bulb-growing areas in the provinces of North- and South-Holland and the North-East Polder in the Netherlands were examined for trichodorid nematodes and tobacco rattle virus (TRV) serotypes. At least one of a total of eight species of Trichodoridae, of which Paratrichodorus pachydermus was most prevalent, was found in 93% of the samples from the provinces of North- and South-Holland and TRV, including four serotypes, was obtained from 49% of these samples. In the North-East Polder one of three species of trichodorids, of which P. teres occurred most frequently, was present in 72% of the samples, and TRV of one serotype was obtained from 28% of these samples. The TRV isolates recovered from these samples reacted serologically with one of four antisera to strains of TRV. Virus transmitted by P. pachydermus reacted to the PRN-, by Trichodorus viruliferus to the RQ-, by P. teres to the N5- and by T. similis, to the TS-antiserum, respectively.