CHANGES IN FILAMENT ACTIN ACCOMPANYING IGE-DEPENDENT AND IGE-INDEPENDENT HISTAMINE-RELEASE FROM IL3-DEPENDENT CULTURED HUMAN BASOPHILS

被引：9

作者：

EBISAWA, M ^{[1
]}

SAITO, H ^{[1
]}

REASON, DC ^{[1
]}

SAKAGUCHI, N ^{[1
]}

KATSUNUMA, T ^{[1
]}

IIKURA, Y ^{[1
]}

机构：

[1] JIKEI UNIV,DEPT PEDIAT,TOKYO 105,JAPAN

来源：

INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY | 1991年 / 94卷 / 1-4期

关键词：

D O I：

10.1159/000235328

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

When cord blood mononuclear cells were cultured in the presence of rhIL-3 for 5 weeks or more, 40-90% of cultured cells became morphologically mature basophils. We analyzed the kinetics of histamine release, changes in filament actin (F-actin), and movement of intracellular Ca2+ ([Ca2+]i) induced by IgE-dependent (anti-IgE) and -independent (fMLP) stimuli in these cultured basophils. Anti-IgE and fMLP released 24.5 +/- 5.4% and 14.5 +/- 4.5% histamine from the cells, respectively. Anti-IgE caused actin polymerization with a peak response at 15 min, which began much later than the elevation of [Ca2+]i. In contrast to anti-IgE stimulation, fMLP induced rapid actin polymerization with a peak response at 30 s in correlation with kinetics of histamine release. Our results indicate that cord blood-derived cultured basophils show similar cell functions to mature basophils, and are useful models with which to investigate the mechanisms of degranulation, specifically when a large amount of highly purified cells are required.

引用

页码：71 / 73

页数：3

共 10 条

[1] PLATELET-ACTIVATING FACTOR-INDUCED ACTIVATION AND CYTOSKELETAL CHANGE IN CULTURED EOSINOPHILS [J].