EVANESCENT FLUOROBIOSENSOR FOR THE DETECTION OF POLYAROMATIC HYDROCARBON BASED ON DNA INTERCALATION

被引：15

作者：

PANDEY, PC ^{[1
]}

WEETALL, HH ^{[1
]}

机构：

[1] NIST,DIV BIOTECHNOL,BIOSENSOR TECHNOL GRP,GAITHERSBURG,MD 20899

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1995年 / 55卷 / 02期

关键词：

EVANESCENT WAVE; POLYAROMATIC HYDROCARBON; PAH; DNA INTERCALATION; BIOSENSOR; FLUOROBIOSENSOR;

D O I：

10.1007/BF02783551

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A flow-injection analysis (FIA) system coupled with an evanescent wave (EW) biosensor employing total internal reflection of fluorescence radiation (TIRF) for the detection of polyaromatic hydrocarbon that intercalates into DNA is reported. A highly fluorescent intercalator, ''ethidium bromide,'' has been used as the reference compound for detection. The EW biosensor was developed according to the procedure described earlier (1,2). Data on the analysis of Naphthalene, 3-methylcholanthrene, 7,12-dimethylbenz(a)anthracene, 1,2-benzanthracene, and some standard reference materials supplied by the National Institute of Standards and Technology are reported. The relative ability of the polyaromatic hydrocarbon to displace ethidium bromide, based on the relative binding ratio, is found to be on the order of 7,12-dimethylbenz[a]anthracene > 3-methylcholanthrene > 1,2-benzanthracene > napthalene.

引用

页码：87 / 94

页数：8

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