EFFECT OF PREINCUBATION PH ON THE GROWTH-CHARACTERISTICS OF AEROMONAS-HYDROPHILA AT 5-DEGREES-C, AS ASSESSED BY 2 METHODS

被引:5
作者
BUNCIC, S
AVERY, SM
机构
[1] Meat Industry Research Institute of New Zealand (Inc.), Hamilton
关键词
D O I
10.1111/j.1472-765X.1995.tb00395.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
TWO strains of Aeromonas hydrophila (the type strain ATCC 7966 and a food-derived strain JAH4) were pre-incubated at 5 degrees C in Brain Heart Infusion (BHI) broth with pH adjusted to 6.0 or 7.0, and then incubated at the same temperature in BHI broth with pH adjusted to 6.0, 6.5, 7.0 and 7.5. Growth kinetics during incubation were determined by two methods: viable count (VC) and measurement of optical density (O.D.). Pre-incubation at different pH values did not significantly affect the maximum specific growth rates of the strains during incubation, but the lag phases were shorter after pre-incubation at pH 6.0 than at pH 7.0. The VC method was more sensitive than O.D. measurements for assessing lag phase.
引用
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页码:7 / 10
页数:4
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