CHRYSAMINE-G BINDING TO ALZHEIMER AND CONTROL BRAIN - AUTOPSY STUDY OF A NEW AMYLOID PROBE

被引：111

作者：

KLUNK, WE ^{[1
]}

DEBNATH, ML ^{[1
]}

PETTEGREW, JW ^{[1
]}

机构：

[1] UNIV PITTSBURGH,SCH MED,WESTERN PSYCHIAT INST & CLIN,DEPT PSYCHIAT,NEUROPHYS LAB,PITTSBURGH,PA 15261

来源：

NEUROBIOLOGY OF AGING | 1995年 / 16卷 / 04期

关键词：

ALZHEIMERS DISEASE; BETA-AMYLOID; NEUROFIBRILLARY TANGLES; CEREBROVASCULAR AMYLOID; DIAGNOSIS; SYNTHESIS; BLOOD-BRAIN BARRIER; DIAZO DYES; CONGO RED; CHRYSAMINE G; HUMAN BRAIN;

D O I：

10.1016/0197-4580(95)00058-M

中图分类号：

R592 [老年病学]; C [社会科学总论];

学科分类号：

03 ; 0303 ; 100203 ;

摘要：

Chrysamine-G (CG) is a carboxylic acid analogue of Congo red, a histologic dye which stains amyloid. CG binds to the beta-amyloid protein of Alzheimer's disease (AD) in vitro and partitions into the brain of normal mice. In this study, we demonstrate increased binding of [C-14]CG to homogenates of several regions of AD brain as compared to control. The total binding of CG to AD brain was approximately two- to three-fold that of control brain. The cerebellum could be used as an internal standard for each brain as CG binding to cerebellum did not differ between AD and control. The binding of [C-14]CG correlated with numbers of senile plaques and neurofibrillary tangles. In addition, CG could be used to stain cerebrovascular amyloid in tissue sections. These results suggest that CG may prove useful as an in vivo probe of amyloid deposition in AD.

引用

页码：541 / 548

页数：8

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