MITOCHONDRIAL AUTONOMY - INCORPORATION OF MONOSACCHARIDES INTO ENDOGENOUS GLYCOLIPID ACCEPTORS IN ISOLATED RAT LIVER MITOCHONDRIA

被引:28
作者
BOSMANN, HB
CASE, KR
机构
[1] Department of Pharmacology, Toxicology University, Rochester Medical School Rochester
关键词
D O I
10.1016/0006-291X(69)90684-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Isolated, sterile rat liver mitochondria homogenized in Triton X-100 incorporated glucose-14C and mannose-14C into endogenous lipid acceptors forming glycolipids. In addition, choline-14C was incorporated into phosphatidyl choline in the system. The glycolipid incorporations were highly specific and showed an absolute requirement for Mg++. One of the products formed with each monosaccharide was identified as a diglyceride glycolipid. Fractionation of the mitochondria into inner and outer membrane fractions indicated that the choline, glucose and mannose systems were associated with the outer membranes of the mitochondria. © 1969.
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页码:830 / &
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