MEASUREMENT OF PLASMA AND URINE AMINO-ACIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION USING PHENYLISOTHIOCYANATE DERIVATIZATION

被引：46

作者：

SHERWOOD, RA ^{[1
]}

TITHERADGE, AC ^{[1
]}

RICHARDS, DA ^{[1
]}

机构：

[1] ROYAL SUSSEX CTY HOSP,DEPT BIOCHEM,BRIGHTON BN2 5BE,E SUSSEX,ENGLAND

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1990年 / 528卷 / 02期

关键词：

D O I：

10.1016/S0378-4347(00)82388-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The use of reversed-phase liquid chromatography (LC) with pre-column derivatization for the analysis of amino acid mixtures is becoming established as a possible cheaper alternative to commercial amino acid analysers. The available derivatization procedures all have disadvantages when applied to clinical samples, partly due to the interferences found with body fluids when ultraviolet or fluorescence detection is used. An LC method is described for the separation of amino acids in blood or urine, using pre-column derivatization with phenylisothiocyanate (PITC), gradient elution and electrochemical detection. The use of electrochemical detection of PITC derivatives virtually eliminates interferences and enables the secondary amino acids to be measured. Examples are shown of normal urine and plasma and samples from patients with cystinuria and maple syrup urine disease. © 1990.

引用

页码：293 / 303

页数：11

共 12 条

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