A SINGLE-CHAIN MURINE CLASS-I MAJOR TRANSPLANTATION ANTIGEN

被引：40

作者：

MOTTEZ, E

JAULIN, C

GODEAU, F

CHOPPIN, J

LEVY, JP

KOURILSKY, P

机构：

[1] INST PASTEUR, INSERM,U277,UNITE BIOL MOLEC GENE,25 RUE DR ROUX, F-75724 PARIS 15, FRANCE

[2] HOP COCHIN, INSERM, U152, F-75674 PARIS 14, FRANCE

来源：

EUROPEAN JOURNAL OF IMMUNOLOGY | 1991年 / 21卷 / 02期

关键词：

D O I：

10.1002/eji.1830210232

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Single-chain mouse K(d) molecules (SC-K(d)) were engineered by connecting residue 276 of K(d) heavy chain to the first residue of beta2-microglobulin through spacers of various lengths, and expressed intracellularly in monkey COS-1 cells. Labeled SC-K(d) molecules were found to react with several monoclonal antibodies which recognize native K(d) molecules. SC-K(d) -15 (with a spacer of 15 residues) was studied in more details. It could be purified and shown to regain a native-like structure after treatment with denaturing agents. Purified SC-K(d)-15 could bind certain peptides in a manner qualitatively similar to that of K(d).

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页码：467 / 471

页数：5

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