CHARACTERISTICS OF THE T-DEPENDENT ALPHA-(1-]6) GLUCOSYL (DEXTRAN) ANTIBODY-RESPONSE INDUCED IN MICE WITH ISOMALTOHEXAOSE COUPLED TO CHICKEN GAMMA-GLOBULIN

Isomaltohexaose flavazole coupled to chicken γ‐globulin (IM6‐CGG) induced T celldependent anti‐α( 1 → 6) dextran‐specific IgM and IgG responses in CBA, BALB/c and A strain mice. The IgG responses were of restricted heterogeneity as judged by isoelectric focusing, and belonged mostly to the IgG1 subclass with a minor IgG3 component in the case of BALB/c and CBA mice. All four subclasses of IgG were produced in A strain mice. In contrast, native dextran B 512 induces exclusively T cell‐independent IgM responses of the same specificity for all 3 strains. All BALB/c mice immunized with different doses of IM6‐CGG either in Freund's adjuvant or with A1(OH)3 plus Bordetella pertussis showed the same spectrotypes by isoelectric focusing. Sera obtained at different times after immunization of BALB/c mice failed to show spectrotype variations. Late, but not early, bleedings from CBA mice showed a tendency towards more uniform isoelectric focusing patterns. Copyright © 1979 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim