SECRETED ANTIGENS OF MYCOBACTERIUM-TUBERCULOSIS - CHARACTERIZATION WITH LYMPHOCYTES-T FROM PATIENTS AND CONTACTS AFTER 2-DIMENSIONAL SEPARATION

被引:39
作者
DAUGELAT, S [1 ]
GULLE, H [1 ]
SCHOEL, B [1 ]
KAUFMANN, SHE [1 ]
机构
[1] UNIV ULM,DEPT IMMUNOL,ALBERT EINSTEIN ALLEE 11,W-7900 ULM,GERMANY
关键词
D O I
10.1093/infdis/166.1.186
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Little is known about T cell antigens involved in immunity against Mycobacterium tuberculosis. Most model systems use in vitro culture of human T lymphocytes with bacterial lysates or secreted proteins as antigens. In this study, proteins from 3-week-old M. tuberculosis culture filtrates were separated by two-dimensional PAGE and subsequently transferred into soluble phase. The resulting 480 fractions were screened with T lymphocytes from tuberculosis patients and healthy contacts. T cells from all 9 patients and from 8 of 10 tuberculin-positive contacts preferentially responded to a cluster of acidic proteins (pI 4-5) with molecular masses of 30-100 kDa, although they also recognized a number of other fractions. In contrast, of 7 tuberculin-negative contacts, 4 were not and 3 were only weakly stimulated by this cluster region. Therefore, this distinct cluster of secreted proteins seems to comprise dominant T cell antigens of M. tuberculosis.
引用
收藏
页码:186 / 190
页数:5
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