SELECTION OF AN EXTRACELLULAR ESTERASE-PRODUCING MICROORGANISM

被引：10

作者：

CASTRO, GR ^{[1
]}

STETTLER, AO ^{[1
]}

FERRERO, MA ^{[1
]}

SINERIZ, F ^{[1
]}

机构：

[1] UNIV BUENOS AIRES,FAC CIENCIAS EXACTAS & NAT,DEPT QUIM BIOL,BUENOS AIRES,ARGENTINA

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY | 1992年 / 10卷 / 3-4期

关键词：

ESTERASE SCREENING TEST; ESTERASE PRODUCTION; BACILLUS-SUBTILIS ESTERASE; TEMPERATURE PROFILE; PH PROFILE;

D O I：

10.1007/BF01569761

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The conventional esterase plate-screening technique has been modified in order to obtain a better differentiation of high producing strains. A short exposure to Lugol's iodine solution after colony growth enhanced the contrast between the precipitation halo and the background. Batch cultures of a selected strain characterized as belonging to the Bacillus subtilis group showed a high level of extracellular activity at pH 6.6 to 8.0 and 35-degrees-C. In crude extracts the optimum enzymatic activity was obtained at 35-degrees-C and pH 7.0.

引用

页码：165 / 168

页数：4

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