SIMPLE METHOD FOR MEASURING THE ANTIBIOTIC CONCENTRATION REQUIRED TO KILL ADHERENT BACTERIA

被引：36

作者：

MIYAKE, Y ^{[1
]}

FUJIWARA, S ^{[1
]}

USUI, T ^{[1
]}

SUGINAKA, H ^{[1
]}

机构：

[1] HIROSHIMA UNIV, SCH MED, HIROSHIMA 734, JAPAN

来源：

CHEMOTHERAPY | 1992年 / 38卷 / 05期

关键词：

BACTERIAL ADHERENCE; ANTIBIOTICS; BACTERICIDAL ACTIVITY;

D O I：

10.1159/000239015

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A simple method was developed for measuring the antibiotic susceptibility of bacteria adherent to plastic surfaces. Staphylococcus aureus cells adhered to the bottom of a 96-well plastic tissue culture plate were incubated in serially diluted antibiotic solutions. After a 24-hour-incubation the solutions were removed, and fresh medium without antibiotics was added to each well. The viability of the cells was judged by their growth after a further 24-hour-incubation. The antibiotic concentration required to kill adherent bacteria was far higher than that required for planktonic cells, although we used bactericidal drugs; beta-lactam, quinolone, and aminoglycoside antibiotics. The tolerance demonstrated by adherent cells is likely to play a role in the difficulties encountered in the chemotherapy of biofilm infections.

引用

页码：286 / 290

页数：5

共 14 条

[1] INTERACTION OF BIOFILM BACTERIA WITH ANTIBIOTICS IN A NOVEL INVITRO CHEMOSTAT SYSTEM [J].