DETECTION OF TRYPANOSOME INFECTIONS IN THE SALIVA OF TSETSE-FLIES AND BUFFY-COAT SAMPLES FROM ANTIGENEMIC BUT APARASITAEMIC CATTLE

被引：75

作者：

MAJIWA, PAO

THATTHI, R

MOLOO, SK

NYEKO, JHP

OTIENO, LH

MALOO, S

机构：

[1] INT CTR INSECT PHYSIOL & ECOL, NAIROBI, KENYA

[2] INT LIVESTOCK CTR AFRICA, KENYA AGR RES INST, MOMBASA, KENYA

来源：

PARASITOLOGY | 1994年 / 108卷

关键词：

DIGOXIGENIN; POLYMERASE CHAIN REACTION; TRYPANOSOMES; TSETSE VECTOR;

D O I：

10.1017/S0031182000076150

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Relatively simple protocols employing non-radioactive DNA probes have been used for the detection of African trypanosomes in the blood of mammalian hosts and the saliva of live tsetse flies. In combination with the polymerase chain reaction (PCR), the protocols revealed trypanosomes in buffy-coat samples from antigenaemic but aparasitaemic cattle and in the saliva of live, infected tsetse flies. Furthermore, the protocols were used to demonstrate concurrent natural infections of single tsetse flies with different species of African trypanosomes.

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页码：313 / 322

页数：10

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