3 METHODS FOR ISOLATING VIABLE ANTHOZOAN ENDODERM CELLS WITH THEIR INTRACELLULAR SYMBIOTIC DINOFLAGELLATES

被引：43

作者：

GATES, RD

MUSCATINE, L

机构：

[1] Department of biology, University of California, Los Angeles, 90024, California

来源：

CORAL REEFS | 1992年 / 11卷 / 03期

关键词：

D O I：

10.1007/BF00255468

中图分类号：

Q17 [水生生物学];

学科分类号：

071004 ;

摘要：

Three maceration methods are described for the isolation of single endoderm cells from marine cnidarians. Two are enzymatic treatments suitable for fleshy anthozoans such as sea anemones and zoanthids. The third employs calcium free sea water and is suitable for stony corals. The viability and morphology of the endoderm cells is described using fluorogenic dyes and scanning and transmission electron microscopy.

引用

页码：143 / 145

页数：3

共 11 条

[1]

DAVID CN, 1983, HYDRA RES METHODS, P1532

[2]

HARVEY HW, 1957, CHEM FERTILITY SEA W, P113

[3]

HERTWIG O, 1879, ACTINIEN ANATOMISCH, P244

[4]

MAURER A, 1989, 5TH INT C COEL BIOL, P85