MOLECULAR-CLONING AND CHROMOSOMAL LOCALIZATION OF THE HUMAN THROMBOPOIETIN GENE

被引：237

作者：

SOHMA, Y

AKAHORI, H

SEKI, N

HORI, T

OGAMI, K

KATO, T

SHIMADA, Y

KAWAMURA, K

MIYAZAKI, H

机构：

[1] KIRIN BREWERY CO LTD,PHARMACEUT RES LAB,MAEBASHI,GUMMA 371,JAPAN

[2] NATL INST RADIOL SCI,DIV GENET,INAGE KU,CHIBA 263,JAPAN

[3] KAZUSA DNA RES INST,GENE STRUCT LAB 1,CHIBA 292,JAPAN

来源：

FEBS LETTERS | 1994年 / 353卷 / 01期

关键词：

THROMBOPOIETIN; GENOMIC DNA; CHROMOSOME MAPPING;

D O I：

10.1016/0014-5793(94)01008-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The complete gene for human thrombopoietin (TPO) has been cloned by screening a human genomic library using human TPO cDNA as a probe. This gene is 6.2 kb in length and contains six exons and five introns. It is shown that the human genome contains a single copy of the human TPO gene according to Southern blotting analysis. The transcription initiation site was determined by S1 nuclease mapping. The human TPO gene expressed TPO activity when transfected into COS-1 cells. The human TPO gene has been mapped to chromosome 3q27 by in situ hybridization using a biotin-labeled probe.

引用

页码：57 / 61

页数：5

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