A ROLE FOR TRANSCRIPTION AND FRGY2 IN MASKING MATERNAL MESSENGER-RNA WITHIN XENOPUS-OOCYTES

被引：178

作者：

BOUVET, P

WOLFFE, AP

机构：

[1] Laboratory of Molecular Embryology National Institute of Child Health, Human Development National Institutes of Health Bethesda

来源：

CELL | 1994年 / 77卷 / 06期

关键词：

D O I：

10.1016/0092-8674(94)90141-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We find that mRNA synthesized within the Xenopus oocyte nucleus is translated with an efficiency 50 times less than that of mRNA injected into the oocyte cytoplasm. For histone H1 mRNA this effect is independent of mRNA splicing, nuclear export, and the promoter driving transcription. The mRNA synthesized in vivo is translationally competent but is masked from the translational machinery in the cytoplasm through association with proteins including frog Y-box protein 2 (FRGY2). We find that overexpression of FRGY2 facilitates the translational repression of mRNA synthesized within Xenopus oocytes. The requirement for transcription to occur in vivo before a translationally repressed state can be established suggests that these two events are functionally coupled in Xenopus oocytes.

引用

页码：931 / 941

页数：11

共 70 条

[1] REPLICATION-COUPLED CHROMATIN ASSEMBLY IS REQUIRED FOR THE REPRESSION OF BASAL TRANSCRIPTION IN-VIVO [J].

ALMOUZNI, G ;

WOLFFE, AP .

GENES & DEVELOPMENT, 1993, 7 (10) :2033-2047

[2] TRANSIENT ACTIVATION OF OOCYTE 5S RNA GENES IN XENOPUS EMBRYOS BY RAISING THE LEVEL OF THE TRANS-ACTING FACTOR TFIIIA [J].

ANDREWS, MT ;

BROWN, DD .

CELL, 1987, 51 (03) :445-453

[3] TRANSCRIPTION FACTOR LOADING ON THE MMTV PROMOTER - A BIMODAL MECHANISM FOR PROMOTER ACTIVATION [J].

ARCHER, TK ;

LEFEBVRE, P ;

WOLFORD, RG ;

HAGER, GL .

SCIENCE, 1992, 255 (5051) :1573-1576

[4] A MATERNAL TAIL OF POLY(A) - THE LONG AND THE SHORT OF IT [J].

BACHVAROVA, RF .

CELL, 1992, 69 (06) :895-897

[5] TAT TRANS-ACTIVATES THE HUMAN IMMUNODEFICIENCY VIRUS THROUGH A NASCENT RNA TARGET [J].

BERKHOUT, B ;

SILVERMAN, RH ;

JEANG, KT .

CELL, 1989, 59 (02) :273-282

[6] XENOPUS-HSP 70 GENES ARE CONSTITUTIVELY EXPRESSED IN INJECTED OOCYTES [J].

BIENZ, M .

EMBO JOURNAL, 1984, 3 (11) :2477-2483

[7] THE DEADENYLATION CONFERRED BY THE 3' UNTRANSLATED REGION OF A DEVELOPMENTALLY CONTROLLED MESSENGER-RNA IN XENOPUS-EMBRYOS IS SWITCHED TO POLYADENYLATION BY DELETION OF A SHORT SEQUENCE ELEMENT [J].

BOUVET, P ;

OMILLI, F ;

ARLOTBONNEMAINS, Y ;

LEGAGNEUX, V ;

ROGHI, C ;

BASSEZ, T ;

OSBORNE, HB .

MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (03) :1893-1900

[8] A NUCLEAR TRANSLATIONAL BLOCK IMPOSED BY THE HIV-1 U3 REGION IS RELIEVED BY THE TAT-TAR INTERACTION [J].

BRADDOCK, M ;

THORBURN, AM ;

CHAMBERS, A ;

ELLIOTT, GD ;

ANDERSON, GJ ;

KINGSMAN, AJ ;

KINGSMAN, SM .

CELL, 1990, 62 (06) :1123-1133

[9] HIV-1 TAT ACTIVATES PRESYNTHESIZED RNA IN THE NUCLEUS [J].

BRADDOCK, M ;

CHAMBERS, A ;

WILSON, W ;

ESNOUF, MP ;

ADAMS, SE ;

KINGSMAN, AJ ;

KINGSMAN, SM .

CELL, 1989, 58 (02) :269-279

[10] RNA-BINDING SPECIFICITY OF HNRNP A1 - SIGNIFICANCE OF HNRNP A1 HIGH-AFFINITY BINDING-SITES IN PRE-MESSENGER-RNA SPLICING [J].

BURD, CG ;

DREYFUSS, G .

EMBO JOURNAL, 1994, 13 (05) :1197-1204

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