PURIFICATION OF URICASE FROM MAMMALIAN TISSUE

被引：13

作者：

CONLEY, TG

PRIEST, DG

机构：

[1] Department of Biochemistry, Medical University of South Carolina Charleston

来源：

PREPARATIVE BIOCHEMISTRY | 1979年 / 9卷 / 02期

关键词：

D O I：

10.1080/00327487908061683

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple, rapid procedure for the purification of uricase from mammalian tissue is reported. The procedure is based on the precipitation of mammalian uricase under certain dialysis conditions, and on its low solubility near neutral pH. Exceptionally high yields of homogeneous enzyme are obtained. © 1979, Taylor & Francis Group, LLC. All rights reserved.

引用

页码：197 / 203

页数：7

共 12 条

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[6] PREPARATION OF URICASE
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[7] Mahler H.R., 1963, ENZYMES, V8, P285
[8] MAHLER HR, 1955, J BIOL CHEM, V216, P625
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PRIEST, DG
FISH, WW
[J]. BIOCHEMISTRY, 1974, 13 (05) : 888 - 892
[10] REACTION INTERMEDIATE EFFECTS ON SPECTROPHOTOMETRIC URICASE ASSAY
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