PURIFICATION OF GLUCOAMYLASE FROM ASPERGILLUS-TERREUS

被引：5

作者：

ALI, S

HOSSAIN, Z

MAHMOOD, S

ALAM, R

机构：

[1] Department of Biochemistry, University of Dhaka, Dhaka

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1990年 / 6卷 / 04期

关键词：

D O I：

10.1007/BF01202129

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Glucoamylase from a rice bran culture of Aspergillus terreus was purified by chromatography on DEAE-cellulose and concanavatin A-Sepharose. A homogenous monomer resulted after SDS-PAGE electrophoresis. The enzyme was a glycoprotein, molecular weight, 86,000 with 7.5% (w/w) carbohydrate content. © 1990 Rapid Communications of Oxford Ltd.

引用

页码：431 / 433

页数：3

共 8 条

[1]

Ali S., Mahmood S., Alam R., Hossain Z., Culture condition for production of glucoamylase from rice bran by Aspergillus terreus, MIRCEN Journal of Applied Microbiology and Biotechnology, 5, pp. 121-127, (1989)

[2]

Dubois M., Gillis K.A., Hamilton J.K., Rebers P.A., Smith F., Colorimetric method for the determination of sugar and related substances, Analytical Chemistry, 28, pp. 350-356, (1956)

[3]

Fogarty W.M., Microbial amylases, Microbial Enzymes and Biotechnology, pp. 1-92, (1983)

[4]

Goldstein I.J., Hollerman C.E., Smith I.E., Protein-carbohydrate interaction—II. Inhibition studies in the interaction of Concanavallin A with polysaccharides, Biochemistry, 4, pp. 876-887, (1965)

[5]

Kanlayakrit W., Ishimatsu K., Nakao M., Hayashida S., Characteristics of raw starch digesting glucoamylase from Rhizomucor pusillus, Journal of Fermentation Technology, 65, pp. 379-385, (1987)

[6]

Laemmili U.K., Cleavage of structural protein during the head of bacteriophage T<sub>4</sub>, Nature (London), 227, pp. 680-685, (1975)

[7]

Manjunath P., Shenoy B.C., Raghuvendra Rao M.R., Fungal glucoamylases, Journal of Applied Chemistry, 5, pp. 235-260, (1983)

[8]

Muthukumuran N., Dhar S.C., Purification and properties of glucoamylase fraction from the culture filtrate of Rhizopus nodusus, Italian Journal of Biochemistry, 32, pp. 239-253, (1983)

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