LYMPHOCYTE MEMBRANE-ALTERATIONS CAUSED BY NYLON WOOL COLUMN SEPARATION

被引：16

作者：

CORRIGAN, A

OKENNEDY, R

SMYTH, H

机构：

[1] Department of Biochemistry, University College, Belfield

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1979年 / 31卷 / 1-2期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0022-1759(79)90298-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Use of the nylon wool column method to separate lymphocytes results in preparations which, while fully viable, have grossly depleted cellular levels of phospholipid, cholesterol and sialic acid. These membrane effects, which relate mainly to T cells, indicate that the method is unsuitable for use in biochemical analysis of lymphocytes. © 1979.

引用

页码：177 / 182

页数：6

共 16 条

[1]

Ames B.N., 1966, METHOD ENZYMOL, V8, P115, DOI [DOI 10.1016/0076-6879(66)08014-5, 10.1016/0076-6879(66)08014-5]

[2]

CHIAMORI N, 1959, AM J CLIN PATHOL, V31, P305

[3] BINDING OF ALPHA-FETOPROTEIN TO MURINE T-CELLS [J].