INVASION OF HEREDITARY OVALOCYTES BY PLASMODIUM-FALCIPARUM INVITRO AND ITS RELATION TO INTRACELLULAR ATP CONCENTRATION

被引:34
作者
DLUZEWSKI, AR
NASH, GB
WILSON, RJM
REARDON, DM
GRATZER, WB
机构
[1] UNIV LONDON KINGS COLL,MRC,MUSCLE & CELL MOTIL UNIT,26-29 DRURY LANE,LONDON WC2B 5RL,ENGLAND
[2] UNIV BIRMINGHAM,SCH MED,DEPT HAEMATOL,BIRMINGHAM B15 2TT,W MIDLANDS,ENGLAND
[3] NATL INST MED RES,LONDON NW7 1AA,ENGLAND
[4] ADDENBROOKES HOSP,DEPT HAEMATOL,CAMBRIDGE CB2 2QQ,ENGLAND
基金
英国医学研究理事会;
关键词
MALARIA; PLASMODIUM-FALCIPARUM; OVALOCYTE; ADENOSINE TRIPHOSPHATE; ERYTHROCYTE METABOLISM;
D O I
10.1016/0166-6851(92)90121-Y
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Hereditary ovalocytes (stomatocytic ovalocytes), when examined within 1-2 days from the time that the blood sample is drawn, are invaded by Plasmodium falciparum in culture to the extent of at least 55% of normal control cells. The ovalocytes have extremely rigid membranes, characterised by a shear elastic modulus some 3-4 times greater than that of normal cells. The extent of invasion falls off very much more rapidly than that into normal cells on storage, and we surmise that this is the reason for earlier reports of resistance of ovalocytes to malarial invasion in vitro. The initial loss of susceptibility to invasion with time is not accompanied by any change in membrane rigidity, but is primarily a consequence of a rapid decline in intracellular ATP concentration: this falls to below the threshold level required for invasion (approx. 0.1 mM) over a period in which the ATP in normal cells remains almost constant. Incubation in a metabolic regenerating medium leads to a rise in the intracellular ATP concentration and invasion by P. falciparum is recovered, though to a much lower extent than in normal cells. The resistance of ovalocytes to invasion becomes irreversible, due possibly to degradative processes in the membrane, on further storage. The developing parasites in ovalocytes have a reduced number of merozoites and show distinct morphological abnormalities.
引用
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页码:1 / 7
页数:7
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