NOVEL HETEROCYCLIC LIGANDS FOR THE THIOPHILIC PURIFICATION OF ANTIBODIES

被引：29

作者：

SCHWARZ, A

KOHEN, F

WILCHEK, M

机构：

[1] WEIZMANN INST SCI,DEPT MEMBRANE RES & BIOPHYS,IL-76100 REHOVOT,ISRAEL

[2] WEIZMANN INST SCI,DEPT HORMONE RES,IL-76100 REHOVOT,ISRAEL

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 664卷 / 01期

关键词：

D O I：

10.1016/0378-4347(94)00404-S

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Novel thiophilic ligands based on mercaptoheterocycles were synthesized for use in one-step purification of antibodies. In order to better characterize these new structures, affinity constants were measured, as well as the influence of pH and salt on adsorption and elution. The ligand concentration was optimized for efficient and fast adsorption and elution of antibodies from ascites and serum. The purification of antibodies from cell culture supernatant proved difficult due the indicator phenol red of the growth media.

引用

页码：83 / 88

页数：6

共 14 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2]

ESHHAR Z, 1982, PROTIDES BIOL FLUIDE, P823

[3] PREPARATION OF IODINE-131 LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC ACTIVITY [J].