DIRECT GAS-CHROMATOGRAPHIC DETERMINATION OF DECHLOROETHYLCYCLOPHOSPHAMIDE FOLLOWING MICROSOMAL INCUBATION OF CYCLOPHOSPHAMIDE

被引：13

作者：

BOHNENSTENGEL, F ^{[1
]}

JOHNSON, S ^{[1
]}

HOFMANN, U ^{[1
]}

EICHELBAUM, M ^{[1
]}

KROEMER, HK ^{[1
]}

机构：

[1] DR MARGARETE FISCHER BOSCH INST CLIN PHARMACOL,D-70376 STUTTGART,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 672卷 / 02期

关键词：

D O I：

10.1016/0378-4347(95)00236-C

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the sensitive determination of dechloroethylcyclophosphamide (3-DCl) in microsomal incubation mixtures was developed. 3-DCl, a side-chain oxidation product of cyclophosphamide (CP), was isolated by extraction with acetic acid ethyl ester following solid-phase extraction on C-8 cartridges. Quantification of the metabolite was performed by direct capillary gas chromatography with a nitrogen-phosphorus detector without prior derivatization. The method showed good sensitivity and reproducibility with a detection limit of 1 ng/ml and a limit of quantification of 5 ng/ml. The suitability of the method is shown for the quantification of 3-DCl following incubation of CP with human liver microsomes.

引用

页码：271 / 276

页数：6

共 16 条

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