CONSTRUCTION OF A SITE-SPECIFIC, DELETION-FRAMESHIFT MUTATION IN AN ESSENTIAL GENE OF BACTERIOPHAGE-PHI-X174

被引：9

作者：

HUMAYUN, MZ

CHAMBERS, RW

机构：

[1] New York University, School of Medicine, Department of Biochemistry, New York, NY 10016

来源：

NATURE | 1979年 / 278卷 / 5704期

关键词：

D O I：

10.1038/278524a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

By in vitro methods, we have deleted 80 nucleotides from a preselected site in the coding sequence of gene G of bacteriophage ΦX174. This mutant (G▽/FS ZH1) also carries a - 2 frameshift. The mutation is lethal, but mutant virus can be stably maintained on host strains bearing plasmids carrying ΦX gene G. © 1979 Nature Publishing Group.

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页码：524 / 529

页数：6

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