GENETIC-ANALYSIS AND OVEREXPRESSION OF LIPOLYTIC-ACTIVITY IN BACILLUS-SUBTILIS

被引：56

作者：

DARTOIS, V ^{[1
]}

COPPEE, JY ^{[1
]}

COLSON, C ^{[1
]}

BAULARD, A ^{[1
]}

机构：

[1] UNIV CATHOLIQUE LOUVAIN, GENET MICROBIENNE LAB, B-1348 LOUVAIN, BELGIUM

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1994年 / 60卷 / 05期

关键词：

D O I：

10.1128/AEM.60.5.1670-1673.1994

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The previously cloned Bacillus subtilis lipase gene (lip) was mapped on the chromosome and used in the construction of a B. subtilis derivative totally devoid of any lip sequence. Homologous overexpression was performed in this strain by subcloning the lip open reading frame on a multicopy plasmid under the control of a strong gram-positive promoter. A 100-fold overproducing strain was obtained, which should facilitate purification of the secreted protein. Furthermore, the Delta lip strain BCL1050 constitutes an ideal host for the cloning of heterologous lipase genes.

引用

页码：1670 / 1673

页数：4

共 16 条

[1] PURIFICATION, GENE CLONING, AMINO-ACID-SEQUENCE ANALYSIS, AND EXPRESSION OF AN EXTRACELLULAR LIPASE FROM AN AEROMONAS-HYDROPHILA HUMAN ISOLATE [J].

ANGUITA, J ;

APARICIO, LBR ;

NAHARRO, G .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (08) :2411-2417

[2]

AUNSTRUP K, 1990, EUR BIOTECHNOL LETT, V94, P3

[3] CLONING, NUCLEOTIDE-SEQUENCE AND EXPRESSION IN ESCHERICHIA-COLI OF A LIPASE GENE FROM BACILLUS-SUBTILIS 168 [J].

DARTOIS, V ;

BAULARD, A ;

SCHANCK, K ;

COLSON, C .

BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1131 (03) :253-260

[4] CONSTRUCTION OF A KIT OF REFERENCE STRAINS FOR RAPID GENETIC-MAPPING IN BACILLUS-SUBTILIS-168 [J].

DEDONDER, RA ;

LEPESANT, JA ;

LEPESANTKEJZLAROVA, J ;

BILLAULT, A ;

STEINMETZ, M ;

KUNST, F .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1977, 33 (04) :989-993

[5] GLYCEROL ESTER HYDROLASE (EC 3.1.1.3) FROM CORYNEBACTERIUM-ACNES - SERINE LIPASE [J].

FULTON, JE ;

NOBLE, NL ;

BRADLEY, S ;

AWAD, WM .

BIOCHEMISTRY, 1974, 13 (11) :2320-2327

[6] BACILLUS-SUBTILIS AND ITS RELATIVES - MOLECULAR BIOLOGICAL AND INDUSTRIAL WORKHORSES [J].

HARWOOD, CR .

TRENDS IN BIOTECHNOLOGY, 1992, 10 (07) :247-256

[7]

HOCH JA, 1991, METHOD ENZYMOL, V204, P305

[8]

IHARA F, 1991, J BIOL CHEM, V266, P18135

[9] SPECIFIC AND SENSITIVE PLATE ASSAY FOR BACTERIAL LIPASES [J].

KOUKER, G ;

JAEGER, KE .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1987, 53 (01) :211-213

[10]

KRAG SS, 1975, J BIOL CHEM, V250, P2813

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