THE 16S-23S RIBOSOMAL-RNA INTERGENIC SPACER REGION OF BARTONELLA (ROCHALIMAEA) SPECIES IS LONGER THAN USUALLY DESCRIBED IN OTHER BACTERIA

被引：47

作者：

ROUX, V ^{[1
]}

RAOULT, D ^{[1
]}

机构：

[1] FAC MED MARSEILLE,CNRS,EP J0054,UNITE RICKETTSIES,F-13385 MARSEILLE,FRANCE

来源：

GENE | 1995年 / 156卷 / 01期

关键词：

BARTONELLA QUINTANA; B-HENSELAE; B-VINSONII; B-ELIZABETHAE; NUCLEOTIDE SEQUENCE; TRNA(ALA); TRNA(LLE);

D O I：

10.1016/0378-1119(94)00919-J

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We amplified by polymerase chain reaction (PCR) and sequenced using an automated laser fluorescent DNA sequencer (Pharmacia) the intergenic spacer region (ITS) between the 16S and 23S rRNAs of the four species of Rochalimaea which were recently renamed Bartonella sp. We obtained DNA fragments of 1211, 1262, 1258 and 1529 bp for the reference species of B. quintana, B. henselae, B. vinsonii and B. elizabethae, respectively. The ITS of the four species are longer than previously reported in prokaryotes and contained the genes encoding isoleucine-tRNA (tRNA(Ile)) and alanine-tRNA (tRNA(Ala)). The sequences of the tRNA(Ala) genes are identical for the four Bartonella species, but the tRNA(Ile) gene sequence of B. quintana presents one mutation in comparison with the other species.

引用

页码：107 / 111

页数：5

共 17 条

[1]

Bacot, Reeves, Novel tRNA gene organization in the 16S-23S intergenic spacer of the Streptococcus pneumoniae rRNA gene cluster, J. Bacteriol., 173, pp. 4234-4236, (1991)

[2]

Brenner, O'Connor, Winkler, Steigerwalt, Proposals to unify the genera Bartonella and Rochalimaea, with descriptions of Bartonella quintana comb. nov., Bartonella vinsonii comb. nov., Bartonella henselae comb. nov., and Bartonella elizabethae comb. nov., and to remove the family Bartonellaceae from the order Rickettsiales, Int. J. Syst. Bacteriol., 43, pp. 777-786, (1993)

[3]

Brosius, Dull, Sleeter, Noller, Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli, J. Mol. Biol., 148, pp. 107-127, (1980)

[4]

Daly, Worthington, Brenner, Moss, Hollis, Weyant, Steigerwalt, Weaver, Daneshvar, O'Connor, Rochalimaea elizabethae sp. nov. isolated from a patient with endocarditis, J. Clin. Microbiol., 31, pp. 872-881, (1993)

[5]

Drancourt, Mainardi, Brouqui, Vandenesch, Carta, Lehnert, Viguier, Goldstein, Acar, Raoult, Bartonella (Rochalimaea) quintana endocarditis in three homeless men., N Engl J Med, (1995)

[6]

Fournier, Ozeki, Structure and organization of the transfer ribonucleic acid genes of Escherichia coli K-12, Microbiol. Rev., 49, pp. 379-397, (1985)

[7]

Frothingham, Wilson, Sequence-based differentiation of strain in the Mycobacterium avium complex, J. Bacteriol., 175, pp. 2818-2825, (1993)

[8]

Hall, Are point mutations or DNA rearrangements responsible for the restriction fragment length polymorphisms that are used to type bacteria, Microbiology, 140, pp. 197-204, (1994)

[9]

Loughney, Lund, Dahlberg, tRNA genes are found between the 16S and 23S rRNA genes in Bacillus subtilis, Nucleic Acids Res., 10, pp. 1607-1622, (1982)

[10]

Lucey, Dolan, Moss, Garcia, Hollis, Weigner, Relapsing illness due to Rochalimaea henselae in immuno competent hosts: implication for therapy and new epidemiological associations, Clin. Infect. Dis., 14, pp. 683-688, (1992)

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