RNA EDITING OF TOBACCO PETB MESSENGER-RNAS OCCURS BOTH IN CHLOROPLASTS AND NONPHOTOSYNTHETIC PROPLASTIDS

被引：22

作者：

HIROSE, T

WAKASUGI, T

SUGIURA, M

KOSSEL, H

机构：

[1] NAGOYA UNIV,CTR GENE RES,NAGOYA,AICHI 46401,JAPAN

[2] UNIV FREIBURG,INST BIOL 3,D-79104 FREIBURG,GERMANY

来源：

PLANT MOLECULAR BIOLOGY | 1994年 / 26卷 / 01期

关键词：

RNA EDITING; NICOTIANA TABACUM; PROPLASTID; CHLOROPLAST; PRE-MESSENGER-RNA;

D O I：

10.1007/BF00039562

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We found an RNA editing site in the protein coding region of tobacco (Nicotiana tabacum) petB transcripts. This editing (C (C) under bar A to C (U) under bar A) leads to an amino acid conversion from proline to leucine. It is observed not only in chloroplasts isolated from tobacco leaves but also in non-photosynthetic proplastids isolated from a tobacco cell culture. Also unspliced pre-mRNA shows complete editing. The editing site is the same as that recently observed in the maize petB transcripts which restores the codon for a highly conserved leucine residue, suggesting that RNA editing at this site is critical for the protein (cytochrome b6) function.

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收藏

页码：509 / 513

页数：5

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