CHARACTERIZATION OF THE SUBGENOMIC RNAS OF AN AUSTRALIAN ISOLATE OF BARLEY YELLOW DWARF LUTEOVIRUS

被引:50
作者
KELLY, L
GERLACH, WL
WATERHOUSE, PM
机构
[1] CSIRO Division of Plant Industry, Canberra, A.C.T. 2601, G.P.O
[2] R.W. Johnson Pharmaceutical Research Institute, Rushcutters Bay, NSW 2011
关键词
D O I
10.1006/viro.1994.1378
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We have characterised the subgenomic RNAs of an Australian isolate of BYDV-PAV. Northern blot analyses of infected plants and protoplasts have shown that this isolate synthesises three subgenomic RNAs. Precise mapping of the transcription start sites of all three subgenomic RNAs and translational analyses of subgenomic RNA 2 and 3 have revealed a number of features. First, the transcription start site of subgenomic RNA 1 in this isolate differs markedly from the start site determined for an Illinois isolate of BYDV-PAV. Second, the start sites of, subgenomic RNA 1 and 2 occur at a sequence that closely resembles the 5' end sequence of the genomic RNA (S'AGUGAAGA). Third, subgenomic RNA 2 appears to express ORF 6 of BYDV-PAV but the gene product is truncated due to the appearance of a new stop codon in the sequence. Last, subgenomic RNA 3, which is abundantly transcribed and encapsidated by the virus particle, appears to have no coding ability. We postulate that this novel subgenomic RNA has a regulatory function. (C) 1994 Academic Press, Inc.
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页码:565 / 573
页数:9
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