A NEW METHOD FOR EXTRACTION OF R-LIPOPOLYSACCHARIDES

被引：1917

作者：

GALANOS, C

LUDERITZ, O

WESTPHAL, O

机构：

[1] Max-Planck-Institut für Immunbiologie, Freiburg, BRD-78, Zähringen

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1969年 / 9卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1969.tb00601.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described which is specific for the extraction of lipopolysaccharides from R form bacteria. The extraction mixture which is monophasic, consists of aqueous phenol, chloroform and petroleum ether. R form lipopolysaccharides (glycolipids). due to their lipophilic nature, are completely soluble in the mixture. S and T form lipopolysaccharides as well as proteins, nucleic acids, and polysaccharides, are insoluble, and they are excluded from the extracts. The method is mild, as it can be carried out at below 10°. The yields are generally higher than those obtained by phenol‐water extraction, and the products are usually water‐soluble. Lipopolysaccharides have been successfully extracted from all R form bacteria so far attempted. Copyright © 1969, Wiley Blackwell. All rights reserved

引用

页码：245 / &

共 15 条

[1]

ASHWELL G, 1965, J BIOL CHEM, V240, P4549

[2] ROUGH MUTANTS OF SALMONELLA TYPHIMURIUM .2. SEROLOGICAL + CHEMICAL INVESTIGATIONS [J].