OPTIMIZED RADIOIMMUNOASSAY OF FVIII RELATED ANTIGEN (F-VIIIR-AG) IN PLASMA AND ELUATES

被引：10

作者：

SAVIDGE, GF ^{[1
]}

CARLEBJORK, G ^{[1
]}

机构：

[1] ST GORANS HOSP, DEPT CLIN CHEM, PAEDIAT CLIN, S-11281 STOCKHOLM, SWEDEN

来源：

THROMBOSIS RESEARCH | 1979年 / 14卷 / 2-3期

关键词：

D O I：

10.1016/0049-3848(79)90245-7

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

A new radioimmunoassay of F VIII-related antigen (F VIIIR:AG) is presented, with optimization based upon the estimation of antibody binding and affinity parameters. The results were calculated using the logit-log transformation, and the assay was shown to be reproducible, accurate and technically easy to perform. Assay sensitivity was found to be 2.8 10-4 units/ml F VIIIR:AG for plasma samples, and 2.8 10-5 units/ml F VIIIR:AG for eluates. Due to its high specificity and precision, the radioimmunoassay was demonstrated to be a valuable diagnostic aid in the identification of the heterozygotic state in asymptomatic von Willebrand's disease. © 1979.

引用

页码：363 / 376

页数：14

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